Translational_Unit

Part:BBa_K1521001:Design

Designed by: Danilo Keiji Zampronio   Group: iGEM14_Brasil-SP   (2014-10-01)


RBS SpoVG (K143021) + lasR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 340
    Illegal AgeI site found at 537
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This protein requires an HSL (homoserine lactone) produced by the gene lasI (C0078) to work appropriately. If you want the lasI is already available with RBS, cds, terminator and a promoter responsive to LasR (K081001).


Source

This part was obtaind through PCR of the part C0079. We also removed the LVA tag and added the RBS SpoVG (K143021).

References